Luria-Delbruck Fluctuation Test
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The rate of mutation of L5178Y asparagine-dependent mouse leukemia cells to asparagine independence and its biological consequences.
LSI78Y mouse lymphoblastic leukemia cells normally require asparagine (ASN) for growth, but phenotypically stable ASN-independent cells are detectable at low fre quency by direct cloning, in ASN-free soft-agar medium, of cells from cultures that have been grown nonselectively in medium containing ASN. The rate of mutation in vitro to ASN independence was determined by the Luria-Delbruck fluctua...
متن کاملThe Rate of Mutation of L5178Y Asparagine-dependent Mouse Leukemia Cells to Asparagine Independence and Its Biological Consequences1
LSI78Y mouse lymphoblastic leukemia cells normally require asparagine (ASN) for growth, but phenotypically stable ASN-independent cells are detectable at low fre quency by direct cloning, in ASN-free soft-agar medium, of cells from cultures that have been grown nonselectively in medium containing ASN. The rate of mutation in vitro to ASN independence was determined by the Luria-Delbruck fluctua...
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A potential drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs is the ease with which viruses may generate escape mutations. Using a variation of the classic Luria-Delbrück fluctuation assay, we estimated that the spontaneous mutation rate in the artificial microRNA (amiR) target of a plant virus was ca. 6 × 10(-5) per replication event.
متن کاملRate of generation of major karyotypic abnormalities in relationship to the metastatic potential of B16 murine melanoma.
To test the hypothesis that genetic instability correlates with malignant potential, we compared the rate of generation of marker chromosomal abnormalities in clones of B16 F1 and B16 F10 murine melanoma. These rates were estimated through an adaptation of fluctuation analysis of Luria and Delbruck (S. E. Luria and M. Delbruck, Genetics, 28: 491-511, 1943). The highly metastatic F10 line showed...
متن کاملHigh-frequency changes in transcriptional activity in polyomavirus-transformed cell lines.
We applied the Luria and Delbruck fluctuation test to analyze high-frequency changes in the phenotype of rat cells transformed by a plasmid carrying the polyomavirus middle T (pmt) gene. All of the transformed cell lines analyzed were capable of switching to the normal state with rates ranging from 10(-3) to 10(-2) per cell per generation. Analysis of both middle T antigen and middle T transcri...
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تاریخ انتشار 2013